Effect of serum on the growth of balb 3T3 A31 mouse fibroblasts and an SV40-transformed derivative

✍ Scribed by James C. Bartholomew; Hisao Yokota; Priscilla Ross

• Publisher: John Wiley and Sons
• Year: 1976
• Tongue: English
• Weight: 620 KB
• Volume: 88
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040880303

✦ Synopsis

Abstract

The effect of serum on the growth properties of non‐transformed Balb 3T3 A31 and SV40‐transformed Balb 3T3 A31 was studied. The concentration of serum in the growth medium of non‐transformed cells had little effect on the initial population doubling time, but did regulate the cell density at which the population became quiescent in G^1^. The doubling time of transformed cells, however, was increased significantly as the concentration of serum was decreased below 4%. This effect on the growth of transformed cells was seen at serum concentrations so low that non‐transformed cells did not complete one population doubling. Flow microfluorometric analysis of these populations indicated that the primary effect of different serum concentrations on the non‐transformed cells was to modulate the average residence time in G^1^; whereas, all the cell cycle phases of the transformed cells were affected by serum. At saturation densities, the non‐transformed cells became quiescent in G^1^, but the transformed cells still traversed the cell cycle and their saturation density appeared to be a balance between cell production and cell death occurring primarily in the G^1^ phase of the cell cycle.