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The effects of biotin and fatty acids on SV3T3 cell growth in the presence of normal calf serum

✍ Scribed by Trudy O. Messmer; Delano V. Young

Publisher: John Wiley and Sons
Year: 1977
Tongue: English
Weight: 442 KB
Volume: 90
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040900212

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The growth of SV3T3 cells in medium containing a low concentration (0.20% v/v) of normal calf serum is enhanced by the addition of biotin or certain unsaturated fatty acids. The biotin effect on the final viable cell density is 5‐ to 10‐fold over the control and is extremely potent, exerting a saturating response at a concentration of approximately 200 pg/ml. The optimal growth response observed with fatty acids is 5‐fold over the control and requires the combination of nervonic acid, palmitoleic acid, and arachidonic acid. The fatty acids are probably not replacing the function of biotin since these two substances are additive in their growth effects.

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