Anchorage-independent growth, i.e., growth in semi-solid medium is considered a marker of cellular transformation of fibroblast cells. Diploid human fibroblasts ordinarily do not exhibit such growth but can grow transiently when medium contains high concentrations of fetal bovine serum. This suggest
โฆ LIBER โฆ
Effect of retinoids on growth factor-induced anchorage independent growth of human fibroblasts
โ Scribed by Helen J. Palmer; Veronica M. Maher; J. Justin Mc Cormick
- Book ID
- 112799918
- Publisher
- Society for In Vitro Biology
- Year
- 1989
- Tongue
- English
- Weight
- 675 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1475-2689
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We have previously observed that a human adrenal carcinoma cell line (SW-13) produces autocrine growth factors that enhance the ability of these cells to clone in soft agar. We now show that a basic FGF-like protein is found in SW-13 lysates and stimulates SW-I3 colony growth. Basic FGF (bFGF) was i