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Enhancement of anchorage-independent growth of a human adrenal carcinoma cell line by endogenously produced basic fibroblast growth factor

✍ Scribed by Shari J. Corin; Lucian C. Chen; Anne W. Hamburger

Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
846 KB
Volume
46
Category
Article
ISSN
0020-7136

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✦ Synopsis

We have previously observed that a human adrenal carcinoma cell line (SW-13) produces autocrine growth factors that enhance the ability of these cells to clone in soft agar. We now show that a basic FGF-like protein is found in SW-13 lysates and stimulates SW-I3 colony growth. Basic FGF (bFGF) was identified in SW-13 lysates by both its chromatographic behavior on heparin-Sepharose and its reactivity with an antibody specific for bFGF. Incubation of lysates with antibodies specific for bFGF abolished their ability to support anchorage-independent growth. Northern analysis demonstrated that the cells produced multiple bFGF mRNA transcripts. SW-I3 cells also expressed bFGF cell-surface recep tors. These data suggest that SW-I3 cells produce and respond to a factor structurally and functionally related to bFGF. Such findings support recent evidence indicating that FGF secreted by human tumor cell lines plays a role in the maintenance of the transformed phenotype.

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