✦ LIBER ✦

Establishment and characterization of TSGH9201, a human gastric carcinoma cell line that is growth inhibited by epidermal growth factor

✍ Scribed by Rong-Yaun Shyu; Shun-Yuan Jiang; Dr. Ming-Yang Yeh; Chang-Chung Wang; Ming-Fang Wu; Horng-Jyh Harn; Tzu-Ming Chang

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 800 KB
Volume: 58
Category: Article
ISSN: 0022-4790
DOI: 10.1002/jso.2930580105

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✦ Synopsis

Abstract

A human signet ring gastric carcinoma cell line TSGH9201 was established in vitro. The cells grew in vitro as a monolayer with polygonal morphology and had a population doubling time of 34 hours. The cells secreted tumor markers CEA and CA 125. They were, however, not tumorigenic in athymic nude mice. Karyotypic analysis demonstrated a near tetraploidy with a modal chromosome number of 98. Northern blotting and immunocytochemical analysis revealed the expression of both transforming growth factor α and high levels of epidermal growth factor receptor. Cell growth was inhibited by the epidermal growth factor in vitro. The cell line may be a useful tool to study autocrine growth regulation through the epidermal growth factor receptor. © 1995 Wiley‐Liss, Inc.

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