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Effect of dietary fat on individual long-chain fatty acyl-CoA esters in rat liver and skeletal muscle

✍ Scribed by Denise M. Ney; John B. Lasekan; Terry Spennetta; Mike Grahn; Earl Shrago

Book ID
112773394
Publisher
Springer-Verlag
Year
1989
Tongue
English
Weight
288 KB
Volume
24
Category
Article
ISSN
0024-4201

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A method for the extraction of acyl-CoA esters from tissue, and their subsequent analysis by HPLC is described. The lipids are removed by a two-phase extraction in a chloroform/methanol/water system. The long-chain acyl-CoA esters are extracted using methanol and a high salt concentration (2 M ammon