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Determination of individual long-chain fatty acyl-CoA esters in heart and skeletal muscle

✍ Scribed by F. Molaparast-Saless; E. Shrago; T. L. Spennetta; S. Donatello; L. M. Kneeland; S. H. Nellis; A. J. Liedtke

Book ID
112773606
Publisher
Springer-Verlag
Year
1988
Tongue
English
Weight
323 KB
Volume
23
Category
Article
ISSN
0024-4201

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A method for the extraction of acyl-CoA esters from tissue, and their subsequent analysis by HPLC is described. The lipids are removed by a two-phase extraction in a chloroform/methanol/water system. The long-chain acyl-CoA esters are extracted using methanol and a high salt concentration (2 M ammon