✦ LIBER ✦

Effect of culture age on the susceptibility of differentiating neuroblastoma cells to retinoid cytotoxicity

✍ Scribed by William S. Kisaalita; John M. Bowen

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 982 KB
Volume: 50
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19960605)50:5<580::aid-bit13>3.0.co;2-n

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✦ Synopsis

The cytotoxic effects of retinoids on neuroblastoma cells at various times during electrophysiological differentiation were evaluated. We used NIE-115, a clone of the murine neuroblastoma C1300 derived from the neural crest, and three retinoids: vitamin A (retinol), all-trans retinoic acid (tretinoin), and 13-cis-retinoic acid (isotretinoin). Differentiating N I E -I 15 cells exposed to retinoids at an isotretinoin ECS0 of 16 p M exhibited the greatest vulnerability in terms of cell death during a period (8 to 10 days) that was previously found to be the most sensitive for induction of gross malformations in rodents. This finding suggested possible similarities between the in vivo and in vitro retinoid mechanism(s) of action. The greatest period of vulnerability to retinoid cytotoxicity was also found to coincide with the rapid resting membrane potential (V,,,) development period, suggesting a linkage between neuronal V,,, and/or electrical excitability development and vulnerability t o retinoid cytotoxicity.

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