## Abstract Epidermal growth factor (EGF) is a mitogen for Swiss 3T3 cells. Short incubation periods with physiological concentrations of EGF induced increased binding of Swiss 3T3 cells to Con Aβcoated nylon fibers. This effect was not induced in an EGF nonβresponsive 3T3 variant, in the transform
Effect of 3T3 plasma membranes on cells exposed to epidermal growth factor
β Scribed by Michael A. Lieberman; Paul Rothenberg; Daniel M. Raben; Luis Glaser
- Book ID
- 118314430
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 442 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0006-291X
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π SIMILAR VOLUMES
Epidermal Growth Factor (EGF) at concentrations of 10(-9) to 10(-10) M initiates cell division in both confluent and low density non-dividing 3T3 cells. Four days after addition of EGF to confluent or low density non-dividing 3T3 cells there is a 2- and 5-fold increase, respectively, in cell number.
Incubation of Swiss mouse 3T3 cells at 37Β°C with bovine brain-derived growth factor (BDGF) decreased the cell surface '251-EGF binding activity of these cells by 70-80%. This down-modulation of the EGF receptor by BDGF was time, temperature, and dose dependent. Scatchard plot analysis indicated that