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DNA Vaccines Volume 29 || Purification of Supercoiled Plasmid

✍ Scribed by Lowrie, Douglas B.; Whalen, Robert


Book ID
124164177
Publisher
Humana Press
Year
1999
Weight
158 KB
Category
Article
ISBN-13
9781592596881

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The interest in purifying injectable-grade plasmid DNA has increased with the development of gene therapy and DNA vaccination technologies. In this paper we develop a method for purifying a 4.8 kb plasmid based on chromatographic processes. An NaCl gradient was optimized on a Q Sepharose 1 column an