Thiophilic interaction chromatography for supercoiled plasmid DNA purification
✍ Scribed by Lena M Sandberg; Åsa Bjurling; Philippe Busson; Jozsef Vasi; Raf Lemmens
- Book ID
- 118471015
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 261 KB
- Volume
- 109
- Category
- Article
- ISSN
- 0168-1656
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The interest in purifying injectable-grade plasmid DNA has increased with the development of gene therapy and DNA vaccination technologies. In this paper we develop a method for purifying a 4.8 kb plasmid based on chromatographic processes. An NaCl gradient was optimized on a Q Sepharose 1 column an
## Abstract Chromatography is one of the key operations in the downstream processing of plasmid DNA (pDNA). However, the increased demand for highly purified pDNA experienced in recent years has made clear the need for alternative processes capable of retaining the advantages of conventional chroma