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DNA Topoisomerase Protocols Volume 94 || Purification of Supercoiled Plasmid DNA

โœ Scribed by Bjornsti, Mary-Ann; Osheroff, Neil


Book ID
111654470
Publisher
Humana Press
Year
1999
Weight
91 KB
Category
Article

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Purification of supercoiled plasmid DNA
โœ G. N. M. Ferreira; J. M. S. Cabral; D. M. F. Prazeres ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 76 KB ๐Ÿ‘ 1 views

The interest in purifying injectable-grade plasmid DNA has increased with the development of gene therapy and DNA vaccination technologies. In this paper we develop a method for purifying a 4.8 kb plasmid based on chromatographic processes. An NaCl gradient was optimized on a Q Sepharose 1 column an