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Extent of supercoiling in plasmid DNA vaccines

✍ Scribed by Dr Manmohan Singh, Mildred Ugozzoli, Elawati Soenawan…

Book ID
120840714
Publisher
Adis International Limited (now part of Wolters Kluwer Health)
Year
2006
Tongue
English
Weight
143 KB
Volume
4
Category
Article
ISSN
1175-9038

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The interest in purifying injectable-grade plasmid DNA has increased with the development of gene therapy and DNA vaccination technologies. In this paper we develop a method for purifying a 4.8 kb plasmid based on chromatographic processes. An NaCl gradient was optimized on a Q Sepharose 1 column an