Ultrafiltration of supercoiled plasmid DNA: Modeling and application
✍ Scribed by António M. Morão; José C. Nunes; Fani Sousa; Maria Teresa Pessoa de Amorim; Isabel C. Escobar; João A. Queiroz
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 944 KB
- Volume
- 378
- Category
- Article
- ISSN
- 0376-7388
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The interest in purifying injectable-grade plasmid DNA has increased with the development of gene therapy and DNA vaccination technologies. In this paper we develop a method for purifying a 4.8 kb plasmid based on chromatographic processes. An NaCl gradient was optimized on a Q Sepharose 1 column an
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