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Isolation of ultrapure supercoiled plasmid-DNA using preparative electrophoresis

✍ Scribed by Dr. Thomas Roeder; Derk Görich; Niklas P. Roeder; Iris Bruchhaus

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
285 KB
Volume
19
Category
Article
ISSN
0173-0835

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✦ Synopsis

Isolation of ultrapure supercoiled plasmid-DNA using preparative electrophoresis

A large-scale preparative polyacrylamide gel electrophoresis (PAGE) system for the isolation of high-purity supercoiled plasmid-DNA is described. This method should prove suitable for the isolation of large DNA molecules, either plasmid or linear DNA, that is required for the production of transgenic animals, for instance. The efficiency of the method is illustrated by the isolation of the gene for the green fluorescent protein, cloned into a mammalian expression vector and used for transfection of eukaryotic cells.

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