DNA-platinum interactions. Characterization of solid DNAK2[PtCl4 complexes

✍ Scribed by J.-P. Macquet; T. Theophanides

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 564 KB
• Volume: 18
• Category: Article
• ISSN: 0020-1693
• DOI: 10.1016/s0020-1693(00)95602-0

✦ Synopsis

A specific reaction of cis-dichlorodiammineplatinum-(II), cis-Pt(NH3& with the GC planes of DNA is reported. This interaction is localized on the N,(G) and the O,(G) sites forming a chelate without proton liberation. On the other hand, the reaction of Kz [PtCl,] with DNA (41% or 72% GC) is also specific and takes place on the GC planes, but the site specificity differs from that of cis-Pt(NH3&lz in that there is proton liberation before the saturation of the N,(G) sites. This may be due to the NIH(G) proton displacement and the formation of an intercrosslink between N,(G) and N3(C). Solid complexes obtained from the reaction of DNA and K,[PtCl,] have been identified and characterized having the formula, Pt (DNA)Clz. The UV spectra of a series of DNA-Pt complexes obtained with K2[PtC1,], K[Pt(C,H.+)Cl,], cis-Pt(en)Cl,, cis-Pt(NHd&, trans-Pt(NH3zC12 and [Pt(dien)CI]

Cl are also reported here. Some thoughts on the mode of action of cis-Pt(NHJ2C12 are presented.