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DNA extraction method from bones using Maxwell® 16

✍ Scribed by Karija Vlahović, Monika; Kubat, Milovan


Book ID
119318110
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
150 KB
Volume
14
Category
Article
ISSN
1344-6223

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Improved DNA extraction from ancient bon
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We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick, QIAGEN). The efficiency of this protoc