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DNA extraction from ancient human bones via enzymatic treatment

โœ Scribed by Carsten Pusch; Michael Scholz


Book ID
114393034
Publisher
Elsevier
Year
1997
Weight
178 KB
Volume
2
Category
Article
ISSN
1366-2120

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We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick, QIAGEN). The efficiency of this protoc