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Ancient DNA extraction from bones and teeth

โœ Scribed by Rohland, Nadin; Hofreiter, Michael


Book ID
109946223
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
201 KB
Volume
2
Category
Article
ISSN
1750-2799

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We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick, QIAGEN). The efficiency of this protoc