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Improved DNA extraction from ancient bones using silica-based spin columns

✍ Scribed by Yang, Dongya Y.; Eng, Barry; Waye, John S.; Dudar, J. Christopher; Saunders, Shelley R.

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 88 KB
Volume: 105
Category: Article
ISSN: 0002-9483
DOI: 10.1002/(sici)1096-8644(199804)105:4<539::aid-ajpa10>3.0.co;2-1

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✦ Synopsis

We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.

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