Disruption of baker's yeast by a new bead mill
β Scribed by Helen Huihua Mao; Murray Moo-Young
- Book ID
- 104647722
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 396 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0951-208X
No coin nor oath required. For personal study only.
β¦ Synopsis
A continuous high-speed bead mill of novel design (Sulzer Annu Mill 01) was tested for cell disruption of baker's yeast as a model system.
The efficiency of cell disruption was evaluated for the relative amount of released protein.
The effects of rotation speed, cell concentration and flow rate of cell suspension on the cell disruption were investigated.
The maximum yield of released protein was found to be 2.62 kg protein/L.h.
This novel design appears to be more effective than existing commercially available mills.
π SIMILAR VOLUMES
Four intracellular enzymes from two species of breadmaking yeasts-S. cerevisiae and C. boidinii-have been measured as a function of time during its disruption using a bead mill in batch operation. The amount and rate of enzyme released was dependent on its location inside the cell as well as on the