A continuous high-speed bead mill of novel design (Sulzer Annu Mill 01) was tested for cell disruption of baker's yeast as a model system. The efficiency of cell disruption was evaluated for the relative amount of released protein. The effects of rotation speed, cell concentration and flow rate of
β¦ LIBER β¦
Optimization of yeast cell disruption with a newly designed bead mill
β Scribed by D. van Gaver; A. Huyghebaert
- Book ID
- 103521812
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 602 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0141-0229
No coin nor oath required. For personal study only.
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Four intracellular enzymes from two species of breadmaking yeasts-S. cerevisiae and C. boidinii-have been measured as a function of time during its disruption using a bead mill in batch operation. The amount and rate of enzyme released was dependent on its location inside the cell as well as on the