Direct Observation of Glycogen Synthesis in Human Muscle with13C NMR
โ Scribed by Thomas Jue, Douglas L. Rothman, Gerald I. Shulman, Bernard A. Tavitian, Ralph A. DeFronzo and Robert G. Shulman
- Book ID
- 123636819
- Publisher
- National Academy of Sciences
- Year
- 1989
- Tongue
- English
- Weight
- 475 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/33716
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## Abstract The integrated ^13^C NMR intensity of the glycogen Cl resonance was measured in skeletal muscle (biceps femoris region) of nine rabbits under __in vivo__ conditions. Concurrent chemical determinations of glycogen content showed that the __in vivo__ signal was 1.02 ยฑ 0.06 the intensity o
## Abstract Recent developments in ^13^C nuclear magnetic resonance (NMR) spectroscopy have permitted noninvasive assessment of glycogen concentration in human skeletal muscle. Before these indirect measurements could be accepted as accurate, it was essential that validation should be carried out b
In vivo NMR has focused on using "P and 'H to study metabolism in humans. Comparable "C NMR studies have not been undertaken, presumably, because of its insensitivity. We report herein that the natural abundance I3C signal from CI glycogen is observable. The ability to observe the signal opens new