Quantification of the glycogen 13C-1 NMR signal during glycogen synthesis in perfused rat liver
β Scribed by N. Bergans; T. Dresselaers; L. Vanhamme; P. Van Hecke; S. Van Huffel; F. Vanstapel
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 228 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0952-3480
- DOI
- 10.1002/nbm.812
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The "C-l NMR peak in proton-decoupled spectra of liver glycogen solution was quantitatively analyzed by three types of model-function fitting algorithms: iterative line-fitting in the frequency domain (MDCON); iterative least-squares fitting (VARF'RO) in the time domain; and noniterative singular va
## Abstract Each of six perfused rat hearts was subjected to 30 min of hypoxia followed by 60 min of reoxygenation. Inversionβrecovery data on the intracellular Na NMR signal, differentiated by a shift reagent, 6 m__M__ Dy(PPP)~2~, were obtained every 5 min, and __T__~1~ values were calculated. The