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Diphotonic one-electron oxidation of NADH on laser excitation at 353 nm

โœ Scribed by Barbara Czochralska; Lars Lindqvist


Book ID
103019205
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
290 KB
Volume
101
Category
Article
ISSN
0009-2614

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โœฆ Synopsis


The prim*a.ry steps in one-electron oxidation of NADH (the reduced form of nicotinamide adenine dinuclcotide) were studied by means of nanosecond laser photolysis combined with kinetic spectrophotometry. High-intensity excitation at 353 nm of NADH in aqueous solution (PH 10.0) was found to produce two-photon ionization at the nicotinamide group. The absorption spectrum of the -NADH+ radical cation formed in thii reaction was determined. This species disappeared by deprotonation at a rate of 3.6 X lo6 s-l at pH 10 forming -NAD.


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