Diphotonic one-electron oxidation of NADH on laser excitation at 353 nm
โ Scribed by Barbara Czochralska; Lars Lindqvist
- Book ID
- 103019205
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 290 KB
- Volume
- 101
- Category
- Article
- ISSN
- 0009-2614
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โฆ Synopsis
The prim*a.ry steps in one-electron oxidation of NADH (the reduced form of nicotinamide adenine dinuclcotide) were studied by means of nanosecond laser photolysis combined with kinetic spectrophotometry. High-intensity excitation at 353 nm of NADH in aqueous solution (PH 10.0) was found to produce two-photon ionization at the nicotinamide group. The absorption spectrum of the -NADH+ radical cation formed in thii reaction was determined. This species disappeared by deprotonation at a rate of 3.6 X lo6 s-l at pH 10 forming -NAD.
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The efficiency of photoelectron ejection from the reduced form of nicotinamide adenine dinucleotide (NADH) in aqueous solution of pH 8.6 was studied as a function of the laser energy by measuring the transient eaq absorption appearing on nanosecond laser excitation at 355 nm. It was found that this
Electron ejection from dihydroflavin mononucleotide was obtained on exposure of this compound in aqueous solution to the third harmonic (355 nm) of a Q-switched Nd/YAG laser. This reaction was studied at different flavin concentrations, laser fluences and laser pulse widths. The results could be acc