Determination of the mechanism of photo-ionization of NADH in aqueous solution on laser excitation at 355 nm

✍ Scribed by Lars Lindqvist; Barbara Czochralska; Ivan Grigorov

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 343 KB
• Volume: 119
• Category: Article
• ISSN: 0009-2614
• DOI: 10.1016/0009-2614(85)85375-6

✦ Synopsis

The efficiency of photoelectron ejection from the reduced form of nicotinamide adenine dinucleotide (NADH) in aqueous solution of pH 8.6 was studied as a function of the laser energy by measuring the transient eaq absorption appearing on nanosecond laser excitation at 355 nm. It was found that this process is due exclusively to consecutive two-photon excitation at the laser energies used. Saturation effects were observed at the higher energies; their origin was discussed. The saturation probably accounts for the results of a recent picosecond study of NADH which seemed to indicate the existence of a monophotonic electron ejection of high quantum yield.