## Quantification of Coomassie Blue Stained Proteins in Polyacrylamide Gels Based on Analyses of Eluted Dye1 Quantification of Coomassie Brilliant Blue R-250 stained proteins on cellulose acetate strips was made over a limited range of protein concentrations (1); this work extended to quantificati
Dimethylformamide interferes with Coomassie dye staining of proteins on blue native gel electrophoresis
โ Scribed by Raghupathy, V.; Oommen, Anna; Ramachandran, Anup
- Book ID
- 122245573
- Publisher
- Elsevier Science
- Year
- 2014
- Tongue
- English
- Weight
- 386 KB
- Volume
- 455
- Category
- Article
- ISSN
- 0003-2697
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A method for the simultaneous staining of proteins during polyacrylamide gel electrophoresis with Coomassie brilliant blue R-250 at pH 2.5 is described. Calf thymus whole histone and cytochrome c were stained by this method and the results obtained were similar to that obtained by staining after ele