## Quantification of Coomassie Blue Stained Proteins in Polyacrylamide Gels Based on Analyses of Eluted Dye1 Quantification of Coomassie Brilliant Blue R-250 stained proteins on cellulose acetate strips was made over a limited range of protein concentrations (1); this work extended to quantificati
Quantitation of proteins on Coomassie blue-stained polyacrylamide gels based on spectrophotometric determination of electroeluted dye
โ Scribed by John M. Malloy; James P. Rieker; Charlene F. Rizzo
- Book ID
- 107711982
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 801 KB
- Volume
- 141
- Category
- Article
- ISSN
- 0003-2697
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A simple method for the extraction of Coomassie brilliant blue R from stained protein bands excised from polyacrylamide gels is described. Spectrophotometric measurement of the eluted dye forms the basis of a sensitive assay to quantitate proteins in gels in the range 0.5-10 micrograms. The method r