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Electrophoresis in the presence of Coomassie brilliant blue R-250 stains polyacrylamide gels during protein fractionation

✍ Scribed by Julian Borejdo; Carroll Flynn

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
489 KB
Volume
140
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method of staining polyacrylamide gels in which the dye is electrophoresed together with the sample is proposed. The method cuts short and simplifies the conventional electrophoresis procedure by eliminating the separate poststaining step. In the gels run in the presence of sodium dodecyl sulfate, the method produces protein staining patterns which are quantitatively identical to the ones obtained by conventional staining procedure. Additional advantages of the method are easy control over the degree of staining and homogenous staining independent of the gel thickness and concentration of the dye.

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