Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions
β Scribed by Guttapadu Sreeramulu; Dr. Nagendra K. Singh
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 417 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0173-0835
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β¦ Synopsis
Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions
π SIMILAR VOLUMES
A method of staining polyacrylamide gels in which the dye is electrophoresed together with the sample is proposed. The method cuts short and simplifies the conventional electrophoresis procedure by eliminating the separate poststaining step. In the gels run in the presence of sodium dodecyl sulfate,
A simple method for the extraction of Coomassie brilliant blue R from stained protein bands excised from polyacrylamide gels is described. Spectrophotometric measurement of the eluted dye forms the basis of a sensitive assay to quantitate proteins in gels in the range 0.5-10 micrograms. The method r
## Quantitation of Submicrogram Amounts of Protein Using Coomassie Brilliant Blue R on Sodium Dodecyl Sulfate-Polyacrylamide Slab-Gels A sodium dodecyl sulfate (SDS)-polyacrylamide slab-gel system was used to study the use of Coomassie brilliant blue (CB) as a quantitative stain. Quantitation curv
A rapid and sensitive protein staining method employing a mixed dye technique has been developed. Solutions of Coomassie brilliant blue R-250 (CB, 0.2%) and Bismark brown R (BBR, 0.05%) were mixed in the volume ratio of 1:0.75 for staining (final molar ratio, 4.5:1). In this staining, BBR inhibits t