𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions

✍ Scribed by Guttapadu Sreeramulu; Dr. Nagendra K. Singh


Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
417 KB
Volume
16
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.

✦ Synopsis


Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions


πŸ“œ SIMILAR VOLUMES


Electrophoresis in the presence of Cooma
✍ Julian Borejdo; Carroll Flynn πŸ“‚ Article πŸ“… 1984 πŸ› Elsevier Science 🌐 English βš– 489 KB

A method of staining polyacrylamide gels in which the dye is electrophoresed together with the sample is proposed. The method cuts short and simplifies the conventional electrophoresis procedure by eliminating the separate poststaining step. In the gels run in the presence of sodium dodecyl sulfate,

Quantitation of proteins by elution of C
✍ Eric H. Ball πŸ“‚ Article πŸ“… 1986 πŸ› Elsevier Science 🌐 English βš– 424 KB

A simple method for the extraction of Coomassie brilliant blue R from stained protein bands excised from polyacrylamide gels is described. Spectrophotometric measurement of the eluted dye forms the basis of a sensitive assay to quantitate proteins in gels in the range 0.5-10 micrograms. The method r

Quantitation of submicrogram amounts of
✍ Richard Kahn; Robert W. Rubin πŸ“‚ Article πŸ“… 1975 πŸ› Elsevier Science 🌐 English βš– 272 KB

## Quantitation of Submicrogram Amounts of Protein Using Coomassie Brilliant Blue R on Sodium Dodecyl Sulfate-Polyacrylamide Slab-Gels A sodium dodecyl sulfate (SDS)-polyacrylamide slab-gel system was used to study the use of Coomassie brilliant blue (CB) as a quantitative stain. Quantitation curv

A Modified Coomassie Blue Staining of Pr
✍ Jung-Kap Choi; Sang-Hyoun Yoon; Hee-Youn Hong; Dong-Ki Choi; Gyurng-Soo Yoo πŸ“‚ Article πŸ“… 1996 πŸ› Elsevier Science 🌐 English βš– 75 KB

A rapid and sensitive protein staining method employing a mixed dye technique has been developed. Solutions of Coomassie brilliant blue R-250 (CB, 0.2%) and Bismark brown R (BBR, 0.05%) were mixed in the volume ratio of 1:0.75 for staining (final molar ratio, 4.5:1). In this staining, BBR inhibits t