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Simultaneous staining of proteins during polyacrylamide gel electrophoresis in acidic gels by countermigration of Coomassie brilliant blue R-250

โœ Scribed by George Varghese; A.M. Diwan

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
739 KB
Volume
132
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A method for the simultaneous staining of proteins during polyacrylamide gel electrophoresis with Coomassie brilliant blue R-250 at pH 2.5 is described. Calf thymus whole histone and cytochrome c were stained by this method and the results obtained were similar to that obtained by staining after electrophoresis.

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