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Dihydroergotoxine: Separation and determination of four components by high-performance liquid chromatography

✍ Scribed by V. Hartmann; M. Rödiger; W. Ableidinger; H. Bethke

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
625 KB
Volume
67
Category
Article
ISSN
0022-3549

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✦ Synopsis

The evaluation of a new high-performance liquid chromatographic method is described. It permits the separation and determination of the four components of dihydroergotoxine (dihydroergocristine, dihydroergocornine, dihydro-alpha-ergocryptine, and dihydro-beta-ergocryptine) in a single step. On reversed-phase microparticles, complete baseline separation is possible with different mobile phases containing about 10(-2) M base. The analysis of dihydroergotoxine mesylate drug substance or its dosage forms can be carried out in about 15 min. No reference substance is required for the determination of the proportions of the components. This method is simple and exhibits high accuracy, reproducibility, and selectivity. It permits the analytical control of dosage forms containing dihydroergotoxine mesylate to ensure that they comply with the specifications for the drug substance used in clinical and pharmacological studies.

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