Differences in the solubilizing effectiveness of the sodium dodecyl sulfate complexes of various proteins
โ Scribed by Steinhardt, Jacinto; Scott, Joan R.; Birdi, Kulbir S.
- Book ID
- 117989216
- Publisher
- American Chemical Society
- Year
- 1977
- Tongue
- English
- Weight
- 1010 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0006-2960
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๐ SIMILAR VOLUMES
A simple method for determination of proteins, initially solubilized in Tris buffer containing sodium dodecyl sulfate (SDS), mercaptoethanol, and sucrose, is described. This method is based on protein and Coomassie brilliant blue G-250 binding but it involves the removal of excess SDS by precipitati
A new, improved method is described, which takes advantage of the low solubility of potassium dodecyl sulfate (KDS), to concentrate sodium dodecyl sulfate-solubilized proteins from nanomolar solutions by KDS precipitation.The method was applied to proteins differing in M, and PI. The effect of varyi