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Fluorescent labeling of proteins in sodium dodecyl sulfate complexes with fluorescamine

✍ Scribed by Shu-I Tu; Leonard Grosso


Book ID
118311028
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
292 KB
Volume
72
Category
Article
ISSN
0006-291X

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A highly sens,tive fluorescent staining method for detecting glycosylated proteins on SDS-polyacrylamide gel has been described. The carbohydrate groups in glycoproteins were oxidized with periodate on SDS-polyacrylamide gel to generate aldehyde groups which were subsequently labeled with a fluoresc