Development of control strategies for high cell density cultivations

✍ Scribed by Reiner Luttmann; Gerhard Bitzer; Jens Hartkopf

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 726 KB
• Volume: 37
• Category: Article
• ISSN: 0378-4754
• DOI: 10.1016/0378-4754(94)00004-2

✦ Synopsis

The high cell density cultivation (HCDC) of genetically modified microorganisms, e.g. E. coli, with dry weight larger than 100 g/l, requires fed-batch processing with aid of a controlled specific growth rate p. A general mathematical model of HCDC as well as different methods to control p is discussed. Furthermore, the implementation of control strategies in an industrial process control system is shortly explained. The test of the control equipment is carried out with by means of on-line simulation methods. The comparison of theoretical investigations with an experimental verification of a high cell density E. coli cultivation demonstrates the the usefulness of our methods.