High-cell-density cultivations of Escherichia coil in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.
High-cell-density cultivation of microorganisms
β Scribed by D. Riesenberg; R. Guthke
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 114 KB
- Volume
- 51
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The commercial interest in functional foods that contain live microorganisms, also named probiotics, is paralleled by the increasing scientific attention to their functionality in the digestive tract. This is especially true of yogurts that contain strains of lacticβacid bacteria of int
The high cell density cultivation (HCDC) of genetically modified microorganisms, e.g. E. coli, with dry weight larger than 100 g/l, requires fed-batch processing with aid of a controlled specific growth rate p. A general mathematical model of HCDC as well as different methods to control p is discuss
## Abstract Using __Cudrania tricuspidata__ cells as model plant cells which have high sensitivity to hydrodynamic stress, technology problems in the cultivation of the plant cells at high density were investigated. Using βshakeβ flasks on a reciprocal shaker and Erlenmeyer flasks on a rotary shake