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Development of a chromatographic method for the determination of saquinavir in plasma samples of HIV patients

✍ Scribed by M. A. Campanero; M. Escolar; M. A. Arangoa; B. Sádaba; J. R. Azanza

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
85 KB
Volume
16
Category
Article
ISSN
0269-3879

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✦ Synopsis

Abstract

A simple, sensitive and reproducible high‐performance liquid chromatographic method for detecting and quantifying saquinavir in human plasma is described. Verapamil was used as internal standard. The method employes a single liquid–liquid extraction step with tert‐butil methyl ether followed by chromatography on a Lichrospher 60 Select B C~8~ reversed‐phase column. Ultraviolet detection was used to identify the compounds of interest. The quantitation limit of saquinavir was 1 ng/mL and only 0.5 mL of plasma sample was required for the determination. The average saquinavir recoveries over a concentration range of 2.5–5000 ng/mL ranged from 86 to 95%. Precision and accuracy did not exceed 5%. Copyright © 2001 John Wiley & Sons, Ltd.

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