High-performance liquid chromatographic method for the determination of mangiferin in rat plasma and urine

✍ Scribed by Hui Wang; Guan Ye; Yi-Hong Tang; Hai-Yan Zhu; Rong-Rong Ma; Zhao-Ling Sun; Cheng-Gang Huang

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 174 KB
• Volume: 20
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.695

✦ Synopsis

A reversed-phase high-performance liquid chromatography assay for mangiferin in rat plasma and urine was developed. Rutin was employed as an internal standard. The mobile phase consisted of acetonitrile-water (16:84, v/v) containing 3% acetic acid at a flow rate of 1 mL/min. Detection was at 257 and 365 nm for mangiferin in plasma and urine, respectively. The limit of quantitation (LOQ) of mangiferin was 0.6 µg/mL in plasma, and 0.48 µg/mL in urine. The standard curve was linear from 0.6 to 24 µg/mL in plasma, and 0.48 to 24 µg/mL in urine, both intra-and inter-day precision of the mangiferin were determined and their RSD did not exceed 10%. The method provides a technique for rapid analysis of mangiferin in rat plasma and urine, which can be used in pharmacokinetic studies.