Development of a method for the determination of danofloxacin in plasma by HPLC with fluorescence detection

✍ Scribed by M. A. Garcia; C. Solans; J. J. Aramayona; S. Rueda; M. A. Bregante

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 49 KB
• Volume: 14
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(200004)14:2<89::aid-bmc931>3.0.co;2-n

✦ Synopsis

A simple and sensitive HPLC method has been developed for the determination of danofloxacin (DAN) in plasma. Sample preparations were carried out by adding phosphate buffer (pH 7.4, 0.1 M), followed by extraction with trichloromethane. DAN and the internal standard, sarafloxacin (SAR), were separated on a reversed-phase column, and eluted with aqueous solutionacetonitrile (80:20 v/v). The fluorescence of the column effluent was monitored at ! ex = 338 and ! em = 425 nm. The retention times were 2.80 and 4.40 min for DAN and SAR, respectively. The method was shown to be linear from 1 to 1500 ng/mL (r 2 = 0.999). The detection and quantitation limit were 1 and 5 ng/mL, respectively. Mean recovery was determined as 80% by the analysis of plasma standards containing 150, 750 and 1500 ng/mL. Inter-and intra-assay precisions were 4.0% and 2.4%, respectively.