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Determination of quercetin in human plasma by HPLC with spectrophotometric or electrochemical detection

✍ Scribed by D. J. L. Jones; C. K. Lim; D. R. Ferry; A. Gescher

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
65 KB
Volume
12
Category
Article
ISSN
0269-3879

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✦ Synopsis

A reversed-phase high-performance liquid chromatographic method for the determination of quercetin in human plasma following intravenous infusion is described. Quercetin in plasma was extracted with methanol-dimethyl sulphoxide (4:1 v/v) and separated on a C18 Hypersil-BDS column with 44% (v/v) methanol in 0.1 M ammonium acetate (pH 5.15) containing 0.27 mM EDTA as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 375 nm, or electrochemically, with a detection limit of 80 ng/mL and 2 ng/mL, respectively.

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