The major pol alpha activity of CHO cells was purified 2 800-fold to near homogeneity and was characterized with respect to its physical and catalytic properties. The purified enzyme, upon analysis in denaturing 'activity' gels, displayed a major, 120 kilodalton, catalytically active core and two mi
Development and characterization of a Chinese hamster ovary cell-specific oligonucleotide microarray
β Scribed by Mark Melville; Padraig Doolan; William Mounts; Niall Barron; Louane Hann; Mark Leonard; Martin Clynes; Tim Charlebois
- Publisher
- Springer Netherlands
- Year
- 2011
- Tongue
- English
- Weight
- 206 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract Stable clones selected for resistance to tunicamycin (TM) have been isolated from Chinese Hamster Ovary (CHO) cells. The TM^R^ phenotype is stable for more than nine months in the absence of the drug. The morphology of TM^R^ mutant varies from epitheloid to abnormally elongate. The muta
## Abstract Stable mutants resistant to an anticancer antibiotic, bleomycinβA2, were selected in Chinese hamster ovary (CHO) cell either spontaneously or after ethylmethane sulfonate mutagenesis. Fluctuation analysis showed that bleomycin resistance occurs in CHO at a rate of 6.50β6.58 Γ 10^β7^ mut
Transfection of Chinese hamster ovary (CHO) cells with a plasmid containing the cDNA for human preprorenin has provided cell lines that secrete 15 mg of native prorenin per liter of culture medium. Tryptic activation of the prorenin occurs by selective cleavage of the Arg66-Leu67 bond (numbering as