Development of apoptosis-resistant dihydrofolate reductase-deficient Chinese hamster ovary cell line

Clonal variability within dihydrofolate

Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: Stability in the absence of selective pressure

✍ No Soo Kim; Sang Jick Kim; Gyun Min Lee 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 344 KB 👁 2 views

Recombinant Chinese hamster ovary (rCHO) cells expressing a high level of chimeric antibody were obtained by cotransfection of heavy-and light-chain cDNA expression vectors into dihydrofolate reductasedeficient CHO cells and subsequent gene amplification in medium containing stepwise increments in m