Determinations of lactate and lactate dehydrogenase activity in serum with the flow injection analysis system involving immobilized enzyme column and chemiluminescence
β Scribed by Masayoshi Tabata; Masayuki Totani; Takashi Murachi
- Book ID
- 107714946
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 620 KB
- Volume
- 193
- Category
- Article
- ISSN
- 0003-2697
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A sensitive flow system for electrochemical determination of the reduced coenzyme NADH was developed using immobilized NADH oxidase from Buciflus lichenifomis. The immobilized enzyme was packed into a 20 mm x 2 mm I.D. mini-column, and the sample volume used was only 5 ~1 per assay, with an assay t