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Japan - Use of a biosensor for determination of serum lactate dehydrogenase activity : In ANAL. CHIM. ACTA (298/1 (113–119) 1994) M. Tabata, F. Koushima, M. Totani of Kyoto University report on “Use of a biosensor consisting of an immobilized NADH oxidase column and a hydrogen peroxide electrode for the determination of serum lactate dehydrogenase activity”

✍ Scribed by Valerie M. Owen

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 195 KB
Volume: 10
Category: Article
ISSN: 0956-5663
DOI: 10.1016/0956-5663(95)96867-x

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Use of a biosensor consisting of an immobilized NADH oxidase column and a hydrogen peroxide electrode for the determination of serum lactate dehydrogenase activity

✍ Masayoshi Tabata; Fumiyo Koushima; Masayuki Totani 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 580 KB

A sensitive flow system for electrochemical determination of the reduced coenzyme NADH was developed using immobilized NADH oxidase from Buciflus lichenifomis. The immobilized enzyme was packed into a 20 mm x 2 mm I.D. mini-column, and the sample volume used was only 5 ~1 per assay, with an assay t