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Determination of validity for blood for transfusion by firefly bioluminescent assay for ATP

✍ Scribed by S. Trajkovska; S. Dzhekova-Stojkova; S. Kostovska

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
241 KB
Volume
290
Category
Article
ISSN
0003-2670

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✦ Synopsis

The duration for which blood used in transfusions is acceptable is an important factor. The validity of blood, conserved and kept at 4"C, has been established by ATP measurement (mol I-') using a rapid firefly bioluminescence method. ATP is extracted from the blood cells with trichloroacetic acid, diluted with Tris buffer (pH 7.75). Samples conserved with CPDA-1 and ACD were compared. Blood stabilised with CPDA-1 was valid for transfusion until the 33rd day, that stabilised with ACD until the 21st day.

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