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Bioluminescent assay of bacterial ATP for rapid detection of bacterial growth in clinical blood cultures

✍ Scribed by Nilsson, L. E. ;Molin, Ö. ;Ånséhn, S.

Publisher
John Wiley and Sons
Year
1989
Weight
333 KB
Volume
3
Category
Article
ISSN
0884-3996

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✦ Synopsis

A bioluminescent assay of bacterial ATP for rapid detection of bacterial growth in 512 clinical aerobic blood cultures was evaluated. At the detection limit of bacterial ATP (lO-'Omol/l) in the blood cultures 94.2% of the true positive blood cultures were detected (sensitivity) and the specificity was 85.8%. If the cut-off limit was increased the sensitivity decreased and the specificity increased and at 2 x 10-9mol/l ATP the maximum correctly classified blood cultures was reached. At this cut-off limit the sensitivity was 82.9% and the specificity was 99.6%. In 54.3% of the true positive blood cultures bacterial growth was detected more rapidly with the bioluminescent assay than with macroscopic examination And subculture.

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