Determination of Urinary L-citrulline by Enzymatic Method
β Scribed by Jiayou Li; Yu Cao; Qian Liu; Qingcai Jiao
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- Chinese
- Weight
- 297 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1872-2040
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L-Citrulline constitutes a product of a number of enzymatic reactions. In the past a number of colorimetric methods for the determination of L-citrulline, upon its chemical modification with diacetyl monoxime at 95Β°C, have been reported. However, all these methods are time-and material-consuming. In
An improved enzymatic method for the determination of pyrroloquinoline quinone, a novel prosthetic group of some important oxidoreductases, has been developed with cytoplasmic membrane of Escherichia coli K-12, in which ~-glucose dehydrogenase (EC 1.1.99.17) was completely resolved to apo-enzyme by