A fluoroenzymatic microdetermination of lactose in tissue culture medium is described. The method is based on the conversion of lactose to galactonic acid by the enzymes galactosidase and galactose dehydrogenase. The resultant reduction of NAD to NADH is measured fluorometrically. Before assaying fo
✦ LIBER ✦
Determination of lactose by an enzymatic method
✍ Scribed by J.A. Sánchez-Manzanares; M.R. Fernández-Villacañas; F. Marín-Iniesta; J. Laencina
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 243 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0308-8146
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