Determination of the molecular size of a plasmid following restriction endonuclease digestion
β Scribed by Peter C Gowland
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 175 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0307-4412
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π SIMILAR VOLUMES
A computer program has been developed for determining the sizes of DNA restriction fragments from their electrophoretic mobiiities. A parabola is fitted to the mobilities of a set of standard fragments of known sizes and the sizes of the unknown fragments are then calculated from the fitted curve. T
The isolation of a new type of gamma transducing phage carrying the bipolar argECBH operon of E. coli K12 is described. The argECBH segment is inserted in the phage in a direction which is opposite from that of previously isolated argECBH-carrying phages. A colE1 argECBH plasmid has been constructed