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A computer program for determining the size of DNA restriction fragments

✍ Scribed by Ronald G. Duggleby; Howard Kinns; Julian I. Rood


Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
466 KB
Volume
110
Category
Article
ISSN
0003-2697

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✦ Synopsis


A computer program has been developed for determining the sizes of DNA restriction fragments from their electrophoretic mobiiities. A parabola is fitted to the mobilities of a set of standard fragments of known sizes and the sizes of the unknown fragments are then calculated from the fitted curve. This procedure is shown to yield estimated sizes which are accurate to within a few percentage, as judged by experiments with fragments obtained by digestion of pBR322 with the restriction endonuclease HaeIII. The program, which is written in BASIC, is simple to use and is very much faster than the graphical method that it replaces.


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